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KMID : 0043319940170060458
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Archives of Pharmacal Research
1994 Volume.17 No. 6 p.458 ~ p.461
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Determination of Omeprazole in Rat Plasma by HPLC with Column Switching
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Shim Sang-Ho
Bok Soo-Jin
Kwon Kwang-II
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Abstract
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A new high-performance liquid chromatographic method with column switching has been onto a Bondapak phenyl/corsil precolumn and polar plasma components were washed with 0.06 M borate burffer. After valve switching, the concentrated drug were eluted in the back-flush mode and separated on a C18 column with acetonitrilke-phosphate buffer as the mobile phae. The method showed excellent precision, accuracy and speed with detection limit of . Total analysis time per smaple was less than 20 min and the coefficients of variation for intra and inter-assay were less than 5.635. This method has been successfully applied to plasma smaples from eats after oral administration of omeprazole.
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KEYWORD
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HPLC, Omeprazole in plasma, Column switching
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